Microbubble-mediated oxygen delivery to hypoxic tissues as a new therapeutic device

Author

Bisazza, Agnese ; Giustetto, Pierangela ; Rolfo, Alessandro ; Caniggia, Isabella ; Balbis, Sonia ; Guiot, Caterina ; Cavalli, Roberta

Author_Institution

Dept Drug Science and Technology, University of Torino, Italy

fYear

2008

fDate

20-25 Aug. 2008

Firstpage

2067

Lastpage

2070

Abstract

Chitosan-coated oxygen microbubbles of average diameter 2.5 μm, narrow size distribution and spherical shape were prepared. A core-shell structure was evidenced by fluorescence microscopy using fluorescent microbubbles. Such microbubbles can be a therapeutic device for vehiculating oxygen to hypoxic tissues, provided they show proper permeability and diffusivity properties and are non-toxic. Our study proves that oxygen is efficiently delivered both in ‘in vitro’ and ‘in vivo’ preparations, and can be conveniently metabolized reversing the cellular hypoxic response. Moreover, toxic effects were investigated in human blood and in cultured cells and no evidence for them was found.

Keywords

Biomedical monitoring; Blood; Cells (biology); Drugs; Fluorescence; Gynaecology; Humans; Neuroscience; Pharmaceutical technology; Transmission electron microscopy; delivery; microbubble; oxygen; release; Animals; Anoxia; Cells, Cultured; Chitosan; Drug Carriers; Eggs; Humans; Hypoxia-Inducible Factor 1, alpha Subunit; Microbubbles; Oxygen;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 2008. EMBS 2008. 30th Annual International Conference of the IEEE

Conference_Location

Vancouver, BC

ISSN

1557-170X

Print_ISBN

978-1-4244-1814-5

Electronic_ISBN

1557-170X

Type

conf

DOI

10.1109/IEMBS.2008.4649599

Filename

4649599