• DocumentCode
    307584
  • Title

    Volumetric image distortion due to refractive index mismatch in 3D confocal scanning laser microscopy

  • Author

    Doukoglou, Tilemachos D. ; Hunter, Ian W.

  • Author_Institution
    Dept. of Biomed. Eng., McGill Univ., Montreal, Que., Canada
  • Volume
    1
  • fYear
    1995
  • fDate
    20-25 Sep 1995
  • Firstpage
    505
  • Abstract
    In the application of 3D-volume reconstruction from 2D image slices acquired by a confocal scanning laser microscope errors arise due to refractive index mismatch. This paper presents a brief theoretical analysis of this error that can lead to miscalculating a specimen´s 3D geometry. A comparison between the error analysis and experimental measurements is also provided
  • Keywords
    biological techniques; biology computing; error analysis; image reconstruction; measurement errors; medical image processing; optical images; optical microscopy; physics computing; refractive index; 2D image slices; 3D confocal scanning laser microscopy; 3D geometry; 3D-volume reconstruction; confocal scanning laser microscope; error analysis; refractive index mismatch; volumetric image distortion;
  • fLanguage
    English
  • Publisher
    ieee
  • Conference_Titel
    Engineering in Medicine and Biology Society, 1995., IEEE 17th Annual Conference
  • Conference_Location
    Montreal, Que.
  • Print_ISBN
    0-7803-2475-7
  • Type

    conf

  • DOI
    10.1109/IEMBS.1995.575222
  • Filename
    575222