Preparation and Structure Analysis of Chondroitin Sulfate from Pig Laryngeal Cartilage

Author

Li, Anlin ; Xiong, Shuangli

Author_Institution

Dept. of Biosci. & Biotechnol., Southwest Univ. of Sci. & Technol., Mianyang, China

fYear

2010

fDate

18-20 June 2010

Firstpage

1

Lastpage

5

Abstract

The chondroitin sulfate with high purity was extracted by papain hydrolysis, isolated and purified by tricloroacetic acid, alcohol precipitation, dialysis and DEAE-Sepharose Fast Flow ion exchange column chromatography. The structure was identified by biological enzymolysis and spectral analysis. GC data exhibited that neutral monosaccharide in chondroitin sulfate only included 0.84% of xylose and 1.28% of galactose. IR data showed that chondroitin sulfate included chondroitin-4-sulfate and chondroitin-6-sulfate isomer in a molecular chain. The results of enzyme hydrolysis and spectra analysis, including reverse phase high performance liquid chromatography, mass-spectrum and NMR, indicated that ChS from pig laryngeal cartilage contains the repeated units of GlcA(β1-3)GalNAc4SO₃ and GlcA(β1-3)GalNAc6SO₃. It may not have the structure characteristics of keratan sulfate and dermatan sulfate such as NeuAc (α2-3) Gal (β1-4) GlcNAc4SO₃ units and GlcA2SO₃ (β1-3) GalNAc4SO₃, GlcA2SO₃ (β1-3) GalNAc6SO₃ units. This may explain the internal mechanism of its antioxidation.

Keywords

biochemistry; biological tissues; biotechnology; enzymes; molecular biophysics; DEAE-Sepharose fast flow ion exchange column chromatography; IR spectra; NMR; alcohol precipitation; biological enzymolysis; chondroitin sulfate; dermatan sulfate; dialysis; high performance liquid chromatography; keratan sulfate; mass spectrum; neutral monosaccharide; papain hydrolysis; pig laryngeal cartilage; spectral analysis; tricloroacetic acid; Animal structures; Biological materials; Biotechnology; Data mining; Isolation technology; Magnetic analysis; Nuclear magnetic resonance; Protection; Purification; Spectral analysis;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioinformatics and Biomedical Engineering (iCBBE), 2010 4th International Conference on

Conference_Location

Chengdu

ISSN

2151-7614

Print_ISBN

978-1-4244-4712-1

Electronic_ISBN

2151-7614

Type

conf

DOI

10.1109/ICBBE.2010.5515812

Filename

5515812