Cloning and Sequence Analysis of a cDNA of P450 17a-Hydroxylase/17,20-Lyase(CYP17) from Strongylocentrotus nudus

Author

Guo, Xiaoli ; Liu, Yang ; Qiu, Xuemei ; Wang, Xiuli

Author_Institution

Coll. of Life Sci. & Biotechnol., Dalian Fisheries Univ., Dalian, China

fYear

2010

fDate

18-20 June 2010

Firstpage

1

Lastpage

4

Abstract

CYP17 (17α-hydroxylase/17, 20-lyase) has been viewed as a critical enzyme for the biosynthesis of gonadal and adrenal steroids in vertebrates. According to a reported CYP17 gene of Strongylocentrotus purpuratus gene from NCBI, three pairs of primers were designed to clone CYP17 gene of Strongylocentrotus nudus,using RT-PCR. Three fragments were assembled into on contig of 1569bp, including full CDS of 1482bp encoding 493 amino acid residues. Sequence analysis indicated the two sea urchin CYP17 genes were mostly similar(97%) . But their amino acid identities were low compared with vertebrates. Cloning of this CYP17 gene would be necessary to characterize its function in development of gonads of sea urchin.

Keywords

DNA; biochemistry; cellular biophysics; enzymes; genetics; genomics; proteomics; CYP17 gene; P450 17a-hydroxylase-17,200-lyase; RT-PCR; Strongylocentrotus Nudus; adrenal steroids; amino acid residues; biosynthesis; cDNA; cloning; enzyme; gonadal steroids; sea urchin; sequence analysis; vertebrates; Amino acids; Aquaculture; Assembly; Biochemistry; Cloning; Encoding; Peptides; Production; RNA; Sequences;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioinformatics and Biomedical Engineering (iCBBE), 2010 4th International Conference on

Conference_Location

Chengdu

ISSN

2151-7614

Print_ISBN

978-1-4244-4712-1

Electronic_ISBN

2151-7614

Type

conf

DOI

10.1109/ICBBE.2010.5516721

Filename

5516721