Effect of ethanol extract concentrations of Hei Chou on calcification of MC3T3-E1 cell

Author

Ahn, Kwang-Sik ; Lee, Young-Hoon ; Kwon, Ha-Jeong ; Park, Yong-duk

Author_Institution

Dept. of Preventive Dentistry, Kyung Hee Univ., Seoul, South Korea

Volume

5

fYear

2010

fDate

16-18 Oct. 2010

Firstpage

1936

Lastpage

1939

Abstract

Some crude drugs have been known to useful effect of bone tissue regeneration in oriental medicine. These drugs were studied many scientific approach for bone formation. In this study, Alizarin Red S assay, and Alkaline phosphatase (ALP) activity of the seed of Hei Chou ethanol extract was investigated to confirm the osteoblastic effect. The result was observed that the seed ethanol extract enhances a significant induction of ALP activity, alizarin red S assay when differentiated osteogenic from MC3T3-E1 cell. In conclusion, Hei Chou extracts increased ALP activity, alizarin red S assay activity for bone formation. These results suggest that Hei Chou acts a successful drug for osteogenic differentiation in vitro and has the potential for use as a therapeutic agent for bone formation.

Keywords

biomedical materials; bone; botany; cellular biophysics; drugs; enzymes; molecular biophysics; Hei Chou ethanol extract concentrations; MC3T3-E1 cell; MC3T3-E1 cell calcification; alizarin red S assay; alkaline phosphatase activity; bone formation; osteoblastic effect; osteogenic cell; osteogenic differentiation in vitro; therapeutic agent; Biomembranes; Bones; Diseases; Drugs; Ethanol; In vitro; Mineralization; Calcification; Hei Chou; MC3T3-E1 Cell; Osteoblast; Pharbitis nil Choisy; component;

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Engineering and Informatics (BMEI), 2010 3rd International Conference on

Conference_Location

Yantai

Print_ISBN

978-1-4244-6495-1

Type

conf

DOI

10.1109/BMEI.2010.5639494

Filename

5639494