Cell-based field effect devices for cell adhesion analysis

Author

Sakata, Tsuyoshi ; Miyahara, Yuji

Author_Institution

Biomater. Center, Nat. Inst. for Mater. Sci., Ibaraki

fYear

2006

fDate

9-12 May 2006

Firstpage

177

Lastpage

179

Abstract

We have developed cell-based field effect devices for cell adhesion analysis. Mouse fibroblast cells were adhered on the Si₃N₄ gate surface of the field effect devices. Attachment and detachment of the cell could be detected as a shift of the flat hand voltage of the field effect devices based on the negative charges of sialic acid at the surface of the cell membrane. Quantitative analysis of cell adhesion could be demonstrated when diluted cell samples are used. The cell-based field effect devices are useful to study cell adhesion characteristics on different surface materials and structures as well as cell functional analysis

Keywords

adhesion; biochemistry; bioelectric phenomena; biological techniques; biomembranes; cellular biophysics; chemical analysis; field effect devices; molecular biophysics; silicon compounds; Si₃N₄; cell adhesion analysis; cell attachment; cell detachment; cell functional analysis; cell membrane surface; cell-based field effect devices; mouse fibroblast cells; negative charges; quantitative analysis; sialic acid; surface materials; Adhesives; Drugs; Electrodes; Fibroblasts; Intersymbol interference; Mice; Optical buffering; Optical microscopy; Silicon compounds; Tellurium; cell attachment; cell-based field effect device; sialic acid;

fLanguage

English

Publisher

ieee

Conference_Titel

Microtechnologies in Medicine and Biology, 2006 International Conference on

Conference_Location

Okinawa

Print_ISBN

1-4244-0337-5

Electronic_ISBN

1-4244-0338-3

Type

conf

DOI

10.1109/MMB.2006.251521

Filename

4281339