Cloning and Expression of a Thermostable Pullulanase Gene from Thermotoga maritima MSB8 in Bacillus subtilis WB600

Author

Su, Zhe ; Lu, Fu-Ping ; Gao, Qiang ; Liu, Xiao-Guang ; Wang, Bin-Zhe ; Niu, Tao

Author_Institution

Key Lab. of Ind. Microbiol., Tianjin Univ. of Sci. & Technol., Tianjin, China

fYear

2010

fDate

18-20 June 2010

Firstpage

1

Lastpage

4

Abstract

The pulA gene enconding a thermostable pullulanase of 844 amino acids was cloned by PCR amplification from the genomic DNA of Thermotoga maritima MSB8. We successfully expressed the pulA gene in a protease-deficient strain Bacillus subtilis WB600, and the expressed pullulanase was secreted into the culture broth. Preliminary results showed that the pullulanase exhibited optimal activity at 90°C, and was stable at the temperature range from 30 to 80°C for 30 min. The stable pH range for this pullulanase was from 5.5 to 7.0, with an optimum at pH 6.0.

Keywords

DNA; biological techniques; cellular biophysics; enzymes; genetics; genomics; molecular biophysics; Bacillus subtilis WB600; PCR amplification; Thermotoga maritima MSB8; amino acids; cloning; culture broth; gene expression; genomic DNA; pH range; protease-deficient strain; pulA gene enconding; temperature 30 degC to 80 degC; temperature 90 degC; thermostable pullulanase gene; time 30 min; Biochemistry; Bioinformatics; Biotechnology; Capacitive sensors; Cloning; DNA; Genomics; Microorganisms; Sequences; Sugar;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioinformatics and Biomedical Engineering (iCBBE), 2010 4th International Conference on

Conference_Location

Chengdu

ISSN

2151-7614

Print_ISBN

978-1-4244-4712-1

Electronic_ISBN

2151-7614

Type

conf

DOI

10.1109/ICBBE.2010.5517795

Filename

5517795