Advanced fluorescence lifetime imaging algorithms for CMOS single-photon sensor based multi-focal multi-photon microscopy

Author

Li, David Day-Uei ; Poland, S. ; Coelho, S. ; Tyndall, D. ; Zhang, Wensheng ; Richardson, J. ; Henderson, Robert K. ; Ameer-Beg, S.M.

Author_Institution

Sch. of Eng. & Inf., Univ. of Sussex, Brighton, UK

fYear

2013

fDate

3-7 July 2013

Firstpage

3036

Lastpage

3039

Abstract

We have developed a fast hardware friendly bi-exponential fluorescence lifetime algorithm suitable for 2D CMOS single-photon avalanche diode (SPAD) arrays. The performance of the proposed algorithm against other techniques is demonstrated on the data from a plant specimen (Convallaria) by using 0.13μm CMOS SPAD arrays mounted on a multi-beam multi-photon microscopy system.

Keywords

CMOS image sensors; avalanche diodes; biomedical optical imaging; fluorescence; optical microscopy; 2D CMOS single-photon avalanche diode arrays; CMOS SPAD arrays; CMOS single-photon sensor; biexponential fluorescence lifetime algorithm; fluorescence lifetime imaging algorithms; multibeam multiphoton microscopy system; multifocal multiphoton microscopy system; plant specimen; Algorithm design and analysis; CMOS integrated circuits; Coordinate measuring machines; Fluorescence; Microscopy; Photonics;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society (EMBC), 2013 35th Annual International Conference of the IEEE

Conference_Location

Osaka

ISSN

1557-170X

Type

conf

DOI

10.1109/EMBC.2013.6610180

Filename

6610180