EGFR-mediated DSB repair pathway in radiosensitization of rectal cancer

Author

Min, Han ; Zhou, Jundong ; Chen, Zhirong ; Hu, Qunchao ; Meng, Xinjun ; Wu, Jinchang ; Zhang, Shuyu

Author_Institution

Dept. of Gastroenterology, Nanjing Med. Univ.focusfocus, Nanjing, China

Volume

2

fYear

2012

fDate

3-5 Aug. 2012

Firstpage

645

Lastpage

651

Abstract

The expression of the epidermal growth factor receptor (EGFR) contributes to radiosensitivity in several cancers, including rectal cancer. However, little is known about the mechanism underlying its radiosensitivity effect. To explore the mechanism, rectal cancer cell line HCT-15 with knockdown of EGFR was established through shRNA. The depletion of EGFR not only inhibited the cell proliferation and focus formation, but also induced cycle blockage of G0/G1 stage and enhanced radiosensitivity. In addition, the mRNA levels of key components involved in NHEJ pathway were significantly down regulated, including Ligase IV, DNA-PKcs and KU 80. EGFR- mediated NHEJ pathway may be involved in rectal cancer radiosensitivity.

Keywords

DNA; cancer; cellular effects of radiation; DNA-PKcs; EGFR mediated DSB repair pathway; HCT-15 cell line; KU 80; Ligase IV; NHEJ pathway; cell proliferation; cycle blockage; epidermal growth factor receptor; radiosensitivity; radiosensitization; rectal cancer; shRNA; Analysis of variance; Cancer; DNA; Educational institutions; Fluorescence; Vectors; EGFR; NHEJ Pathway; Radiosensitivity; rectal cancer;

fLanguage

English

Publisher

ieee

Conference_Titel

Information Technology in Medicine and Education (ITME), 2012 International Symposium on

Conference_Location

Hokodate, Hokkaido

Print_ISBN

978-1-4673-2109-9

Type

conf

DOI

10.1109/ITiME.2012.6291388

Filename

6291388