Preparation and biological performance evaluation of Lactoferrin/ Nanosized hydroxyapatite/ Collagen I composite material

Author

Ma, Ning ; Xu, Wen-zhou ; Zhang, Li ; Lan, Albert ; Jin, Ming-guang ; Liu, Xue-min ; Ying, Di

Author_Institution

Sch. of Stomatology, JiLin Univ., Changchun, China

Volume

2

fYear

2012

fDate

3-5 Aug. 2012

Firstpage

655

Lastpage

659

Abstract

Objective: To prepare and analyse Lactoferrin/Nanosized hydroxyapatite/Collagen I (CMs-loaded LF/nHA/Co-I) composite materials. Methods: Tripolyphosphate (TPP) was used as cross-linker to make CMs-loaded LF. nHA was prepared through chemical precipitation. Collagen I (Co- I) was extracted from fresh bovine tendon through acid dissolution and protease digestion. CMs, nHA and collagen could be crosslinked by Genipin to be tissue engineering materials. The composite materials would be tested by scanning electron microscopy (SEM), enzyme-linked immunosorbent assay (ELISA), immunohistochemistry, X-ray analysis, infrared spectroscopy, average bone density X-ray and analysis by computer software. Results: Genipin could effectively crosslink CNs, nHA and collagen together to form engineering material to repair bone defect.

Keywords

X-ray analysis; bioceramics; biomedical measurement; bone; calcium compounds; infrared spectra; nanocomposites; nanofabrication; nanomedicine; nanoparticles; precipitation (physical chemistry); proteins; scanning electron microscopy; CM loaded LF-nHA-Co-I; Ca₅(PO₄)₃(OH); ELISA; SEM; TPP cross linker; X-ray analysis; acid dissolution; biological performance evaluation; bone defect repair; bone density; bovine tendon; chemical precipitation; composite material preparation; enzyme linked immunosorbent assay; genipin cross linker; immunohistochemistry; infrared spectroscopy; lactoferrin-nanosized hydroxyapatite-collagen I; protease digestion; scanning electron microscopy; tissue engineering materials; tripolyphosphate cross linker; Acoustics; Chitosan; Collagen; DIGORA Optime Intraoral dental film digital imaging systems; Lactoferrin; Microsphere; Nanosized hydroxyapatite; Scanning electron microscope; X-ray; infrared spectral analysis;

fLanguage

English

Publisher

ieee

Conference_Titel

Information Technology in Medicine and Education (ITME), 2012 International Symposium on

Conference_Location

Hokodate, Hokkaido

Print_ISBN

978-1-4673-2109-9

Type

conf

DOI

10.1109/ITiME.2012.6291390

Filename

6291390