Noise reduction in fluorescence Optical Projection Tomography

Author

Darrell, A. ; Marias, K. ; Brady, M. ; Meyer, H. ; Birk, U. ; Ripoll, J.

Author_Institution

Inst. of Comput. Sci., Found. for Res. & Technol.-Hellas (FORTH), Heraklion

fYear

2008

fDate

10-12 Sept. 2008

Firstpage

56

Lastpage

59

Abstract

Optical Projection Tomography (OPT) suffers from additive CCD detector noise. This paper investigates the nature of the CCD detector noise for an OPT setup. The CCD detector noise is found to be described well by an intensity dependent zero-mean Gaussian distribution. A method of reducing the effect of noise in fluorescence OPT reconstructions is discussed. The results of the noise reduction are demonstrated using both artificial scenes created with fluorescent microspheres and real auto-fluorescence OPT projections of the cuticle of the amphipod crustacean Parhyale hawaiensis.

Keywords

CCD image sensors; Gaussian distribution; bio-optics; cellular biophysics; fluorescence; optical noise; optical projectors; optical tomography; CCD detector noise; Gaussian distribution; Parhyale hawaiensis; fluorescence; microspheres; noise reduction; optical projection tomography; Additive noise; Charge coupled devices; Detectors; Fluorescence; Gaussian distribution; Gaussian noise; Noise reduction; Optical noise; Optimized production technology; Tomography; Fluorescence; Noise; Optical Projection Tomography; Tomography;

fLanguage

English

Publisher

ieee

Conference_Titel

Imaging Systems and Techniques, 2008. IST 2008. IEEE International Workshop on

Conference_Location

Crete

Print_ISBN

978-1-4244-2496-2

Electronic_ISBN

978-1-4244-2497-9

Type

conf

DOI

10.1109/IST.2008.4659940

Filename

4659940