Virus enrichment for single virus manipulation by using 3D insulator based dielectrophoresis

Author

Masuda, Taisuke ; Maruyama, Hisataka ; Honda, Ayae ; Araf, Fumihito

Author_Institution

Dept. of Micro-Nano Syst. Eng., Nagoya Univ., Nagoya, Japan

fYear

2011

fDate

15-18 Aug. 2011

Firstpage

241

Lastpage

244

Abstract

We developed an active virus filter (AVF) that was able to virus enrichment for single virus manipulation by using the insulator based dielectrophoresis (iDEP). The design of 3D constricted flow channel enabled the microfluidic chip to produce iDEP force. We utilized maskless photolithography to achieve the precise 3D gray-scale exposure for constricted flow channel. When we applied sinusoidal wave to the electrodes within the range of amplitude of 20 Vp-p and frequency of 10 MHz. AVF achieved enrichment of APR/8 influenza virus by the negative dielectrophoretic force. These results demonstrated that AVF have the dual function of the virus trapping and the virus adherence inhibition.

Keywords

bioMEMS; bioelectric phenomena; biological specimen preparation; electrophoresis; microfluidics; microorganisms; 3D constricted flow channel design; 3D gray scale exposure; 3D insulator based dielectrophoresis; APR/8 influenza virus enrichment; AVF; active virus filter; electrode sinusoidal wave; frequency 10 MHz; iDEP force; maskless photolithography; microfluidic chip; negative dielectrophoretic force; single virus manipulation; virus adherence inhibition; virus trapping; voltage 20 V; Conferences; Government; Nanotechnology; USA Councils;

fLanguage

English

Publisher

ieee

Conference_Titel

Nanotechnology (IEEE-NANO), 2011 11th IEEE Conference on

Conference_Location

Portland, OR

ISSN

1944-9399

Print_ISBN

978-1-4577-1514-3

Electronic_ISBN

1944-9399

Type

conf

DOI

10.1109/NANO.2011.6144622

Filename

6144622