Positioning and levitation media for the separation of biological cells

Author

Arnold, W. Michael

Author_Institution

Gracefield Res. Centre, Ind. Res. Ltd., Lower Hutt, New Zealand

Volume

4

fYear

1999

fDate

1999

Firstpage

2470

Abstract

In response to the requirement for highly polarizable liquid media for electric-field mediated manipulation of microparticles, solutions of highly polar substances are characterised in terms of their permittivity and other characteristics. Requirements for dielectrophoretic levitation and the generalised electromanipulation of biological cells and other microparticles are considered. Examples of the positioning and separation of biological cells are given. Cell separations at the micro and macro scales, the latter using the recently-developed technique of lev-vection, are presented. Finally, growth and division of yeast cells during long-term exposure to the electric fields necessary for this type of separation is reported

Keywords

biological techniques; electric fields; electrostatic devices; position control; biological cells separation; dielectrophoretic levitation; electric-field mediated microparticle manipulation; electromanipulation; highly polar substances; highly polarizable liquid media; lev-vection; levitation media; permittivity; positioning media; separation; yeast cells; Biological cells; Conductivity; Dielectrophoresis; Electrodes; Frequency; Fungi; Geometry; Levitation; Permittivity; Polarization;

fLanguage

English

Publisher

ieee

Conference_Titel

Industry Applications Conference, 1999. Thirty-Fourth IAS Annual Meeting. Conference Record of the 1999 IEEE

Conference_Location

Phoenix, AZ

ISSN

0197-2618

Print_ISBN

0-7803-5589-X

Type

conf

DOI

10.1109/IAS.1999.799187

Filename

799187