Dual-probe luminescence lifetime measurements for the oxygen compensation in enzymatic biosensors

An optical system for monitoring glucose with a means of compensating for local oxygen variations is described. Measurements of sensor response to glucose under varying oxygen levels indicate a strong dependence of the optical response on ambient oxygen. The two-probe approach enables simultaneous monitoring of luminescence lifetime of microparticle sensors with a compact optical system. Glucose tracking experiments with both probes reveal a modulation of oxygen in the vicinity of sensors, even when external oxygen is constant. These results prove the concept of simultaneous monitoring of multiple sensor types in the same matrix.