Assayed Sperm Damnification by SCGE and Its Correlation with Sperm Motility and Sperm Malformation Rate

Author

Xue Baigong ; Yao zhanfei

Author_Institution

Norman Bethune Coll. of Med., Jilin Univ., Changchun, China

fYear

2011

fDate

10-12 May 2011

Firstpage

1

Lastpage

4

Abstract

Objectives: To investigate the role played by sperm damnification in reproduction and correlation with sperm motility and sperm malformation rate. Method: We chosed 186 clinical samples for assaying their semen parameters by Computer Assisted Semen Analysis (CASA), meanwhile, we investigated sperm DNA damnification by Single cell gel electrophoresis(SCGE). Our work highlight that the correlation analysis of DNA damnification with the sperm deformity and motility. Then, we randomly selected some datas made statistics analysis by SPSS13.0. Result: The results showed that the SCGE method is very accurate for detected sperm DNA damnification. Statistics analysis showed that compared with the control group (male fertility), sperm viability, DNA damnification and morphology are significant difference of the group low sperm motility (P <;0.05).

Keywords

DNA; bioelectric phenomena; bioinformatics; cell motility; correlation methods; electrophoresis; statistical analysis; Computer Assisted Semen Analysis; DNA morphology; SCGE; assayed sperm damnification; correlation analysis; single cell gel electrophoresis; sperm malformation rate; sperm motility; sperm viability; statistical analysis; Correlation; DNA; Head; Hospitals; Injuries; Morphology;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioinformatics and Biomedical Engineering, (iCBBE) 2011 5th International Conference on

Conference_Location

Wuhan

ISSN

2151-7614

Print_ISBN

978-1-4244-5088-6

Type

conf

DOI

10.1109/icbbe.2011.5780103

Filename

5780103