DocumentCode
3321493
Title
Notice of Retraction
Purification and Bioactivity of the Angiogenesis Inhibitor DCAIF-II from the Cartilage of Dasyatis akajei: Angiogenesis Inhibitor DCAIF-II from the Cartilage of Dasyatis akajei
Author
Bin Wang ; Gang Feng ; Hong-yu Luo ; You-Le Qu
Author_Institution
Sch. of Food & Pharmacy, Zhejiang Ocean Univ., Zhoushan, China
fYear
2011
fDate
10-12 May 2011
Firstpage
1
Lastpage
4
Abstract
Notice of Retraction
After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE´s Publication Principles.
We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper.
The presenting author of this paper has the option to appeal this decision by contacting TPII@ieee.org.
Guided by anti-angiogenesis activity, a new angiogenesis inhibitor DCAIF-II was isolated from the cartilage of Dasyatis akajei. By phosphate buffer extraction, refrigerated centrifugation, ultrafiltration, 30%-60% ammonium sulphate precipitation, dialysis and refrigerated desiccation, the fraction DCCE with higher anti-angiogenesis active was obtained from the cartilage of D. akajei. Using ion exchange chromatography and gel filtration chromatography, DCAIF-II was purified from DCCE and homogeneous as a single band of 58.2 kDa in 12% SDS-PAGE stained with coomasie brilliant blue. The bioactivity of the DCAIF-II was investigated by inhibiting the formation of the blood vessels in the chick embryo chorioallantoic membrane (CAM). The ratio between the vascular area and the non vascular area (VA/NVA) in sampling point was used for quantitative analysis of the inhibitory effect of DCAIF-II. Compared with the control group (phosphate buffer and chondroitine sulfate), the sampling point of the DCAIF-II group had significantly lower VA/NVA. A large area of blood vessels in sampling point was significantly faded and the vascular structure was blurred with broken branches accompanied by the decreased density of vessels. The finding indicated that the DCAIF-II from the cartilage of D. akajei had an angiogenesis inhibitory effect, and there was also a positive correlation between the concentration and the inhibitory effect.
After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE´s Publication Principles.
We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper.
The presenting author of this paper has the option to appeal this decision by contacting TPII@ieee.org.
Guided by anti-angiogenesis activity, a new angiogenesis inhibitor DCAIF-II was isolated from the cartilage of Dasyatis akajei. By phosphate buffer extraction, refrigerated centrifugation, ultrafiltration, 30%-60% ammonium sulphate precipitation, dialysis and refrigerated desiccation, the fraction DCCE with higher anti-angiogenesis active was obtained from the cartilage of D. akajei. Using ion exchange chromatography and gel filtration chromatography, DCAIF-II was purified from DCCE and homogeneous as a single band of 58.2 kDa in 12% SDS-PAGE stained with coomasie brilliant blue. The bioactivity of the DCAIF-II was investigated by inhibiting the formation of the blood vessels in the chick embryo chorioallantoic membrane (CAM). The ratio between the vascular area and the non vascular area (VA/NVA) in sampling point was used for quantitative analysis of the inhibitory effect of DCAIF-II. Compared with the control group (phosphate buffer and chondroitine sulfate), the sampling point of the DCAIF-II group had significantly lower VA/NVA. A large area of blood vessels in sampling point was significantly faded and the vascular structure was blurred with broken branches accompanied by the decreased density of vessels. The finding indicated that the DCAIF-II from the cartilage of D. akajei had an angiogenesis inhibitory effect, and there was also a positive correlation between the concentration and the inhibitory effect.
Keywords
biological techniques; biomembranes; blood vessels; chromatography; purification; ultrafiltration; zoology; Dasyatis akajei cartilage; ammonium sulphate precipitation; angiogenesis inhibitor DCAIF-II; angiogenesis inhibitory effect; anti-angiogenesis activity; bioactivity; blood vessels; chick embryo chorioallantoic membrane; chondroitine sulfate; coomasie brilliant blue; dialysis; gel filtration chromatography; ion exchange chromatography; non-vascular area; phosphate buffer; phosphate buffer extraction; purification; refrigerated centrifugation; refrigerated desiccation; ultrafiltration; vascular area; vascular structure; Blood vessels; Computer aided manufacturing; Inhibitors; Proteins; Purification; Sediments; Tumors;
fLanguage
English
Publisher
ieee
Conference_Titel
Bioinformatics and Biomedical Engineering, (iCBBE) 2011 5th International Conference on
Conference_Location
Wuhan
ISSN
2151-7614
Print_ISBN
978-1-4244-5088-6
Type
conf
DOI
10.1109/icbbe.2011.5780244
Filename
5780244
Link To Document