Ultrashort pulse electroporation: applications of high pulsed electric fields to induced caspase activation of human lymphocytes

Author

Gundersen, Martin ; Vernier, P. Thomas ; Marcu, Laura ; Li, Aimin ; Zhu, Xhumei ; Gallam, Ali Z. ; Katsouleas, Thomas ; Young, Clayton ; Behrend, Matthew ; Craft, Cheryl M.

Author_Institution

Dept. of Elec. Eng.-Electrophys., Univ. of Southern California, Los Angeles, CA, USA

fYear

2002

fDate

30 June-3 July 2002

Firstpage

667

Lastpage

670

Abstract

Presents evidence for apoptosis, or programmed cell death, and up-regulation of a subset of important genes induced by intense electric fields of short duration (10´s nsec). The fields are observed to perturb mitochondrial membranes and the compartmentalized intracellular environment of Jurkat T lymphocytes, and within hours caspase activation occurs. Intense, but low energy, fields can penetrate a biological cell and invoke mechanisms associated with apoptosis.

Keywords

bioelectric phenomena; biological effects of fields; biomembranes; cellular effects of radiation; genetics; Jurkat T lymphocytes; apoptosis; biological cell; caspase activation; compartmentalized intracellular environment; gene subset; high pulsed electric fields; human lymphocytes; intense electric fields; intense low energy fields; mitochondrial membranes; programmed cell death; short duration; ultrashort pulse electroporation; up-regulation; Biological cells; Biological tissues; Biomedical engineering; Biomembranes; Cells (biology); Circuit simulation; EMP radiation effects; Electromagnetic heating; Electromagnetic radiation; Humans;

fLanguage

English

Publisher

ieee

Conference_Titel

Power Modulator Symposium, 2002 and 2002 High-Voltage Workshop. Conference Record of the Twenty-Fifth International

ISSN

1076-8467

Print_ISBN

0-7803-7540-8

Type

conf

DOI

10.1109/MODSYM.2002.1189565

Filename

1189565