Microfabricated addressable particles for multianalyte immunoassay

Micromachining techniques have been employed to manufacture metallic microparticles bearing an engraved bit-type signature for bimolecular encoding. The well-defined metallic microparticles are, with proper organic derivatization of the surfaces, used for coupling antibodies and served as a platform in the carrying out of high-throughput multiplexed binding assays based on suspension array technology. As proof-of-concept, we demonstrated that encoded microparticles immobilized with anti-IgA, anti-IgG, and anti-IgM antibodies, could be used to sensitively and specifically detect the corresponding antigens in a parallel manner using imaging detection technique.