DocumentCode
3342052
Title
Notice of Retraction
Expression and Purification of Non-Structural Protein NS1 of Human Bocavirus and Generation of Its Antibody
Author
Bin Sun ; Pengying Yue ; Jingjing Li ; Hu Han ; Jinfeng Ouyang ; Yingyue Cai ; Yongbo Yang ; Yi Li
Author_Institution
Coll. of Life Sci., Huazhong Normal Univ., Wuhan, China
fYear
2011
fDate
10-12 May 2011
Firstpage
1
Lastpage
4
Abstract
Notice of Retraction
After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE´s Publication Principles.
We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper.
The presenting author of this paper has the option to appeal this decision by contacting TPII@ieee.org.
Human bocavirus (HBoV) was first discovered in 2005 in nasopharyngeal aspirates of children with respiratory tract infection. To further investigate functions of NS1 of the HBoV, we need badly the NS1 antibody, therefore, we used a prokaryotic expression system pMAL-c2x vector for the production of NS1 as a fusion protein with maltose binding protein (MBP). The target fusion protein which exhibited a strong reaction with anti-MBP antibody was purified by Amylose affinity chromatography to immunize the New Zealand white rabbit for preparing the corresponding antibody. The titer of the produced antibody was measured by ELISA. It was found by using Western blot analysis that polyclonal antibodies were specific against both the NS1 and MBP.
After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE´s Publication Principles.
We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper.
The presenting author of this paper has the option to appeal this decision by contacting TPII@ieee.org.
Human bocavirus (HBoV) was first discovered in 2005 in nasopharyngeal aspirates of children with respiratory tract infection. To further investigate functions of NS1 of the HBoV, we need badly the NS1 antibody, therefore, we used a prokaryotic expression system pMAL-c2x vector for the production of NS1 as a fusion protein with maltose binding protein (MBP). The target fusion protein which exhibited a strong reaction with anti-MBP antibody was purified by Amylose affinity chromatography to immunize the New Zealand white rabbit for preparing the corresponding antibody. The titer of the produced antibody was measured by ELISA. It was found by using Western blot analysis that polyclonal antibodies were specific against both the NS1 and MBP.
Keywords
biochemistry; cellular biophysics; chromatography; microorganisms; molecular biophysics; pneumodynamics; proteins; New Zealand white rabbit; Western blot analysis; amylose affinity chromatography; human bocavirus; maltose binding protein; nasopharyngeal aspirates; nonstructural protein NS1; polyclonal antibodies; prokaryotic expression system; respiratory tract infection; target fusion protein; Bioinformatics; Biomembranes; Biotechnology; DNA; Humans; Proteins; Purification;
fLanguage
English
Publisher
ieee
Conference_Titel
Bioinformatics and Biomedical Engineering, (iCBBE) 2011 5th International Conference on
Conference_Location
Wuhan
ISSN
2151-7614
Print_ISBN
978-1-4244-5088-6
Type
conf
DOI
10.1109/icbbe.2011.5781326
Filename
5781326
Link To Document