One-Step Pathogen Specific DNA Extraction from Whole Blood on a Centrifugal Microfluidic Device

Author

Cho, Yoon-Kyoung ; Lee, Jeong-Gun ; Park, Jong-Myeon ; Lee, Beom-Seok ; Lee, Youngsun ; Ko, Christopher

Author_Institution

Samsung Adv. Inst. of Technol., Suwon

fYear

2007

fDate

10-14 June 2007

Firstpage

387

Lastpage

390

Abstract

We could, for the first time, demonstrate a fully integrated pathogen specific DNA extraction from whole blood utilizing centrifugal microfluidics on a polymer based CD platform. By combining the TS-LIMBS (target separation and laser-irradiated magnetic bead system) and centrifugal microfluidics using the novel LIFM (the laser irradiated ferrowax microvalves), DNA extraction experiments from whole blood spiked with Hepatitis B virus (HBV) were conducted. The total process was finished within 12 minutes with only one manual step of loading 100 muL of whole blood. Real-time PCR results showed that the concentration of DNA prepared on a CD was as good as that of the samples prepared in conventional bench top method.

Keywords

DNA; bioMEMS; biomechanics; biomedical equipment; blood; microfluidics; microorganisms; microvalves; molecular biophysics; Hepatitis B virus; blood; centrifugal microfluidic device; pathogen specific DNA extraction; polymer based CD platform; real-time PCR; target separation and laser-irradiated magnetic bead system; the laser irradiated ferrowax microvalves; Blood; DNA; Liver diseases; Magnetic separation; Microfluidics; Microvalves; Pathogens; Petroleum; Plasma materials processing; Polymers; CD; DNA extraction; laser; microvalve; pathogen;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems Conference, 2007. TRANSDUCERS 2007. International

Conference_Location

Lyon

Print_ISBN

1-4244-0842-3

Electronic_ISBN

1-4244-0842-3

Type

conf

DOI

10.1109/SENSOR.2007.4300149

Filename

4300149