Whole-Cell Conductometric Biosensor for Determination Heavy-Metals in Water

Author

Berezhetskyy, A.L. ; Durrieu, C. ; Chovelon, J.-M. ; Dzyadevych, S.V. ; Tran-Minh, C.

fYear

2007

fDate

10-14 June 2007

Firstpage

1421

Lastpage

1424

Abstract

Whole-cell Chlorella vulgaris conductometric biosensors consisting of interdigitated gold electrodes and sol-gel algal membranes have been used for assessment of heavy-metal ions in water. These analytes act as algal alkaline phosphatase (AP) inhibitors. Enzyme residual activity has been measured in Tris-nitrate buffer in the presence of Mg²⁺ ions as activator. The results indicate that inhibition of AP was found for ppm concentration levels of tested metals while activation of AP activity occurs at ppb concentration levels, except for Zn²⁺ optimized and its characteristics are discussed.

Keywords

biosensors; cellular biophysics; electrochemical electrodes; inhibitors; magnesium; sol-gel processing; water; Chlorella vulgaris; Mg²⁺; algal alkaline phosphatase inhibitors; enzyme residual activity; gold electrodes; heavy-metal ions; sol-gel algal membranes; water; whole-cell conductometric biosensor; Algae; Biochemistry; Biomembranes; Biosensors; Electrodes; Environmentally friendly manufacturing techniques; Frequency measurement; Inhibitors; Pollution measurement; Sensor phenomena and characterization; Chlorella vulgaris; conductometry; heavy-metals ion determination; inhibitor analysis; sol-gel immobilization; thin-film interdigitated electrodes;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems Conference, 2007. TRANSDUCERS 2007. International

Conference_Location

Lyon

Print_ISBN

1-4244-0842-3

Electronic_ISBN

1-4244-0842-3

Type

conf

DOI

10.1109/SENSOR.2007.4300410

Filename

4300410