Multi-stage, solvent-controlled routing for automated on-disc extraction of total RNA from breast cancer cell line homogenate

Author

Gaughran, J. ; Dimov, N. ; Clancy, E. ; Barry, T. ; Smith, T.J. ; Ducree, J.

Author_Institution

Biomed. Diagnostics Inst., Dublin City Univ., Dublin, Ireland

fYear

2013

fDate

16-20 June 2013

Firstpage

305

Lastpage

308

Abstract

We report for the first time rotationally controlled extraction of total RNA from cell homogenate through novel, solvent-dependent routing on a centrifugal micro-fluidic platform. This system allows for sample preparation and integrated analysis to be incorporated on a single microfluidic device. Also reported here for the first time is a solvent-triggered valving mechanism and the performance of on-disc extraction of purified total RNA.

Keywords

RNA; biomedical engineering; cancer; cellular biophysics; microfluidics; automated on-disc extraction; breast cancer cell line homogenate; centrifugal microfluidic platform; multistage routing; single microfluidic device; solvent-controlled routing; solvent-triggered valving mechanism; total RNA; Educational institutions; Liquids; Microfluidics; Protocols; RNA; Routing; Valves; Total RNA extraction; centrifugal microfluidics; solvent dependent routing; solvent triggered valving;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS & EUROSENSORS XXVII), 2013 Transducers & Eurosensors XXVII: The 17th International Conference on

Conference_Location

Barcelona

Type

conf

DOI

10.1109/Transducers.2013.6626763

Filename

6626763