An approach to ex vivo expansion of stem cells

Author

Roehlecke, Uwe ; Brethauer, Kurt ; Ehninger, Gerhard ; Poll, Ruediger

Author_Institution

Inst. of Biomed. Eng., Tech. Univ. Dresden, Germany

Volume

1

fYear

1999

fDate

1999

Abstract

Presented paper describes experimental works within the development of a new bioreactor which is specialized on hematopoietic stem cells. Preceding experiments with static suspension cultures and feeder layer-supported cultures were used to find the optimal media consistence and culture conditions. Perfused cultures with reproducible courses of experiments were proved to obtain higher expansion rates. The process parameters and special optical parameters were determined. The research on experimental setup is useful for further clinical applications

Keywords

biological techniques; blood; cellular biophysics; bioreactor; ex vivo expansion; feeder layer-supported cultures; hematopoietic stem cells; optimal media culture conditions; perfused cultures; process parameters; static suspension cultures; umbilical cord blood; Biomedical engineering; Biomedical measurements; Biomedical optical imaging; Bioreactors; Blood; Cells (biology); Monitoring; Optical sensors; Pensions; Stem cells;

fLanguage

English

Publisher

ieee

Conference_Titel

[Engineering in Medicine and Biology, 1999. 21st Annual Conference and the 1999 Annual Fall Meetring of the Biomedical Engineering Society] BMES/EMBS Conference, 1999. Proceedings of the First Joint

Conference_Location

Atlanta, GA

ISSN

1094-687X

Print_ISBN

0-7803-5674-8

Type

conf

DOI

10.1109/IEMBS.1999.802147

Filename

802147