Diamagnetic accumulation and alignment of nucleoprotein microparticles in high gradient magnetic field

Magnetic force acting on single micron and submicron biological particles having diamagnetic susceptibility in the respect to solution caused their movement and accumulation in gradient magnetic fields. Methods developed allowed the obtaining of magnetic moment values of single particles and their assembly directly from magnetic separation image analysis not assuming to detect sizes. Our precision methods of the measurements of capture traveling time and accumulation radius, in high gradient magnetic separation (HGMS) analysis have shown the determined shifts of magnetic susceptibility up to 0.5·10^-9 (CGS). Image analysis of the concentrating process of nucleoprotein granules (micro-cells, or DNA-granules) with polarization microscopy revealed phase transitions for DNA, and separation accumulation of particles allowed the determination of the diamagnetic moments and anisotropy properties.