Endothelial cell responses to controlled pulsatile flow at the bifurcation of a model vessel

Author

Okada, Masahiko ; Sugita, Osamu ; Miida, Takashi ; Makino, Yuko ; Komoro, Noriko

Author_Institution

Sch. of Med., Niigata Univ., Japan

fYear

1994

Firstpage

291

Abstract

The authors exposed endothelial cells of the porcine aorta to different flow conditions using a model vessel. This model is made of glass tube and consists of a main tube and a side branch of which angle is 90°. The shear rate of the main tube was 20 sec^-1, while that of the side branch was 29 sec^-1. Lactic dehydrogenase (LDP) was measured as an indicator of cell damage. Changes of LDH from cultured cells in dishes by adding native lipoprotein, oxidized and non-oxidized linoleic acid were also examined for comparisons. LDH was lower in the cells of the side branch than in those of the main tube

Keywords

cellular biophysics; haemorheology; pulsatile flow; cell damage indicator; controlled pulsatile flow; endothelial cell responses; flow conditions; glass tube model; lactic dehydrogenase; model vessel bifurcation; native lipoprotein; nonoxidized linoleic acid; oxidized linoleic acid; porcine aorta; Adhesives; Arteries; Atmospheric modeling; Bifurcation; Biochemistry; Cells (biology); Cities and towns; Glass; Laboratories; Muscles; Reservoirs;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 1994. Engineering Advances: New Opportunities for Biomedical Engineers. Proceedings of the 16th Annual International Conference of the IEEE

Print_ISBN

0-7803-2050-6

Type

conf

DOI

10.1109/IEMBS.1994.412039

Filename

412039