Characteristic of arrayed flexible glucose biosensor integrated with the microfluidic device

In this study, the arrayed flexible glucose biosensor modified by graphene (GR) and integrated with the microfluidic device was developed. In order to prepare the glucose biosensor, graphene solution was first cast on the ruthenium dioxide (RuO₂) electrode which were fabricated by radio frequency sputtering and screen-printed technique. Then, the composite of glucose oxidase (GOx) and nafion was dropped on RuO₂ to complete preparation of the RuO₂/Graphene/GOx-Nafion glucose biosensor. Finally, the modified glucose biosensor was combined with the microfluidic device to implement the glucose measurement at different flow rates from 5 μL/min to 30 μL/min. The experimental results showed that the RuO₂/Graphene/GOx-Nafion glucose biosensor had higher sensitivity at 15 μL/min optimum flow rate, and the average sensitivity and linearity were 11.647 mV/mM and 0.995, respectively. Compare with other glucose biosensor measured at static conditions, the results demonstrated that the glucose biosensor had superior performance at the dynamic measurement.