General purpose segmentation for microorganisms in microscopy images

In this paper, we propose an approach for achieving generalized segmentation of microorganisms in microscopy images. It employs a pixel-wise classification strategy based on local features. Multilayer perceptrons are utilized for classification of the local features and is trained for each specific segmentation problem using supervised learning. This approach was tested on five different segmentation problems in bright field, differential interference contrast, fluorescence and laser confocal scanning microscopy. In all instance good results were achieved with the segmentation quality scoring a Dice coefficient of 0.831 or higher.