Photonic Crystal Fiber Surface Plasmon Resonance Biosensor Based on Protein G Immobilization

Author

Wei Chang Wong ; Chi Chiu Chan ; Jia Liang Boo ; Zi Yong Teo ; Zhi Qiang Tou ; Hong Bin Yang ; Chang Ming Li ; Kam Chew Leong

Author_Institution

Div. of Bioeng., Nanyang Technol. Univ., Singapore, Singapore

Volume

19

Issue

3

fYear

2013

fDate

May-June 2013

Firstpage

4602107

Lastpage

4602107

Abstract

A gold-coated photonic crystal optical fiber (PCF) sensor has been proposed as a surface plasmon resonance biosensor. The sensor was fabricated by collapsing the holes of PCF at both ends, causing the light from the multimode fiber to reach the cladding/gold interface for surface plasmon excitation. Mixed thiol and protein G coating were employed on the gold surface, which improved the orientation of the IgG leading to enhanced performance. The sensor was able to monitor the binding kinetics of the IgG (anti-IgG) complexes and a minimum detection limit at a concentration of 0.267 mg/L of anti-IgG was found.

Keywords

biosensors; fibre optic sensors; gold; holey fibres; optical fibre fabrication; photonic crystals; proteins; surface plasmon resonance; Au; PCF; multimode fiber; photonic crystal fiber; protein G immobilization; surface plasmon excitation; surface plasmon resonance biosensor; Gold; Optical fiber sensors; Optical fibers; Proteins; Refractive index; Sensitivity; Surface treatment; Biosensor; optical fiber sensor; photonic crystal fiber (PCF); protein G; surface plasmon resonance (SPR);

fLanguage

English

Journal_Title

Selected Topics in Quantum Electronics, IEEE Journal of

Publisher

ieee

ISSN

1077-260X

Type

jour

DOI

10.1109/JSTQE.2013.2244560

Filename

6425403