An AFM-based study of porcine OBP film for artifical olfaction

Author

Cai, Qiang ; Pan, Min ; Li, Guang ; Chen, Yuquan

Author_Institution

Biosensor Nat. Special Lab, Zhejiang Univ., China

Volume

4

fYear

2001

fDate

2001

Firstpage

3440

Abstract

The odorant binding protein (OBP) plays an important role in olfactory signal transmission. This paper studies the potential application of OBP on bionic artificial nose. Above all, OBP is immobilized in solid phase. So two kinds of OBP film were prepared. Firstly, OBP is covalently immobilized on the silicon wafer using DITC as a crosslinker. Secondly, polypyrrole film was fabricated on the ITO glass by the electrochemical polymerization. The topograph of these films was measured using an Atomic Force Micrograph (AFM). In order to evaluate the affinity of OBP and its antibody, the AFM probe was coated with the anti-OBP using the covalent method. The force-distance curve, acquired by the AFM instrument, was analyzed to reveal the characteristics of thin film and the binding property between OBP and antibody.

Keywords

atomic force microscopy; biosensors; chemioception; electrochemistry; gas sensors; proteins; thin film devices; AFM-based study of porcine OBP film; ITO glass; aqueous mucus barrier; artifical olfaction; binding property; covalently immobilized proteins; crosslinker; force-distance curve; hydrophobic odorous molecules; lipocalin family; odorant-binding protein; olfactory sensory neuron; rupture force; silicon wafer; soluble proteins; thin film; Atomic measurements; Force measurement; Glass; Indium tin oxide; Nose; Olfactory; Polymer films; Proteins; Silicon; Solids;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 2001. Proceedings of the 23rd Annual International Conference of the IEEE

ISSN

1094-687X

Print_ISBN

0-7803-7211-5

Type

conf

DOI

10.1109/IEMBS.2001.1019569

Filename

1019569