Optical biopsy of lung adenocarcinoma using multiphoton fluorescence and harmonic generation microscopy

Lung tumor is a major disease in Taiwan and the development of minimally invasive imaging to detect and diagnose lung tumor in vivo is of biomedical value. However, prior to in vivo investigation, it is important to obtain the multiphoton signatures associated with lung tumor ex vivo. In this investigation, we used multiphoton microscopy to image normal and cancerous lung tissues (adenocarcinoma). Our results show that a combination of harmonic generation and multiphoton autofluorescence imaging may be used to acquire structural information in discerning lung carcinoma from normal lung tissues.