DocumentCode
500000
Title
3D fluorescent particle tracking with nanometer scale accuracies using a double-helix point spread function
Author
Pavani, Sri Rama Prasanna ; Piestun, Rafael
Author_Institution
Dept. of Electr. & Comput. Eng., Univ. of Colorado at Boulder, Boulder, CO, USA
fYear
2009
fDate
2-4 June 2009
Firstpage
1
Lastpage
2
Abstract
We demonstrate parallel three-dimensional (3D) tracking of multiple fluorescent microspheres with nanometer scale accuracies by engineering the 3D point spread function of a wide-field microscope to present a double-helix along the optical axis.
Keywords
fluorescence; nanophotonics; optical microscopy; optical tracking; optical transfer function; particle tracks; 3D double-helix point spread function; multiple fluorescent microsphere; nanometer scale; parallel 3D particle tracking; wide-field microscope; Biomedical optical imaging; Fluorescence; Focusing; Nanobioscience; Optical imaging; Optical microscopy; Optical modulation; Optical scattering; Optical signal processing; Particle tracking; (110.1758) Computational imaging; (180.6900) Three-dimensional microscopy; (280.7250) Velocimetry;
fLanguage
English
Publisher
ieee
Conference_Titel
Lasers and Electro-Optics, 2009 and 2009 Conference on Quantum electronics and Laser Science Conference. CLEO/QELS 2009. Conference on
Conference_Location
Baltimore, MD
Print_ISBN
978-1-55752-869-8
Electronic_ISBN
978-1-55752-869-8
Type
conf
Filename
5225378
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