Effect of mechanical stress on formation of osteoblast in vitro — A new way to culture osteoblasts

Author

Song, Wang ; Yueqin, Yang ; Guoqing, Chen

Author_Institution

Dept. of Human Sports Sci., Wuhan Inst. of Phys. Educ., Wuhan, China

Volume

3

fYear

2009

fDate

8-9 Aug. 2009

Firstpage

432

Lastpage

460

Abstract

Objective: To find a new way to culture osteoblasts. Methods: Rat mesenchymal stem cells (MSCs) were cultured with mechanical stress by (XX) 10 mins a day for seven days. Giemsa staining was used to tell the shapes of the cells and alizarin red S staining was used to detect the formation of osteoblasts. Results: (1) The shapes of the cells suffered with the mechanical stress were not as the same as the MSCs. (2) A few mineralization nodes were detected after cultured with the mechanical stress. Conclusion: Culturing MSCs with mechanical stress is a new way to obtain the osteoblasts.

Keywords

biomechanics; biotechnology; bone; tissue engineering; alizarin red S staining; giemsa staining; mechanical stress; mesenchymal stem cells; osteoblast; time 10 min; time 7 day; Bones; Cells (biology); Extracellular; Humans; In vitro; Mineralization; Proteins; Shape control; Stress control; Tensile stress; MSCs; Mechanical stress; Osteoblasts;

fLanguage

English

Publisher

ieee

Conference_Titel

Computing, Communication, Control, and Management, 2009. CCCM 2009. ISECS International Colloquium on

Conference_Location

Sanya

Print_ISBN

978-1-4244-4247-8

Type

conf

DOI

10.1109/CCCM.2009.5267860

Filename

5267860