Application of Near Infrared Spectroscopy in Rapid Determination of Adenosine and Polysaccharide in Cordyceps Militaris

The adenosine and polysaccharide contents in cordyceps militaris were analyzed with near infrared spectroscopy (NIRS) by establishing models with partial least square (PLS). Varies pretreating spectra methods were used for conversion of NIRS in order to remove the noise in the spectra. The pretreated spectra were applied to develop PLS quantitative analysis models respectively. Each model was optimized by selecting the most suitable amount of factors. The optimal model was selected depend on the root mean squares of calibration sets calculate by cross-validation (RMSECV) and the root mean squares of predicted sets (RMSEP). The RMSECV values of the optimum PLS models for determination adenosine and polysaccharide contents were 0.7592 and 0.0093 respectively. Using this optimum model for determination of adenosine and polysaccharide contents in cordyceps militaris mycelium powder, The RMSEP values were 0.6720 and 0.0083 respectively.