Skin Damage of Ablative Laser in Vivo Based on Multiphoton Microscopy

Recently, various lasers therapies have been employed in clinical practice. And the lasers which relied on their wide clinical applications in treating cutaneous diseases and performing cosmetic procedures have developed greatly. The effects in treatment of laser treatment depend on the immediate response of incident light irradiated in tissues and then the subsequent tissue regeneration and remodeling, especially ablative lasers. In this study, multiphoton microscopy with autofluorescence and second harmonic generation has developed to monitor the optic-thermal response of skin tissue irradiated by CO₂ and the process of regeneration. The injury dimensions are also obtained in the experiment. Our findings show that the method has potential ability to monitor the process of tissue change and the remodeling in mouse dermis. The outcome indicates that the injury dimension and area increase with irradiated dose, and the injury threshold is 0.5W. And then the renovation time also increases with laser fluence.