A membrane capacitance measurement system for the study of exocytosis in secretory cells

Author

Shaughnessy, Thomas J O ; Kim, Yong I.

Author_Institution

Depts. of Biomed. Eng. & Neurology, Univ. of Virginia Health Sci. Center, Charlottesville, VA, USA

Volume

1

fYear

1992

fDate

Oct. 29 1992-Nov. 1 1992

Firstpage

97

Lastpage

98

Abstract

A system for measuring exocytotic secretion in a single cell was developed utilizing an IBM compatible 386 PC. With sinusoidal excitation of the cells, a software based phase detection technique was implemented to monitor exocytosis-induced changes in membrane capacitance. The system is capable of monitoring exocytosis in response to either calcium or depolarizing voltage stimulation. The system has proven to be a useful tool in the study of changes in exocytosis produced by drugs and neurological disorders.

Keywords

bioelectric potentials; biomedical measurement; biomembrane transport; calcium; capacitance measurement; drugs; medical disorders; medical signal processing; neurophysiology; Ca; IBM compatible 386 PC; depolarizing voltage stimulation; drugs; exocytosis; exocytotic secretion; membrane capacitance measurement system; neurological disorders; phase detection; secretory cells; sinusoidal cell excitation; Capacitance; Filtering theory; Monitoring; Noise measurement; Software; Timing; Voltage measurement;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 1992 14th Annual International Conference of the IEEE

Conference_Location

Paris

Print_ISBN

0-7803-0785-2

Electronic_ISBN

0-7803-0816-6

Type

conf

DOI

10.1109/IEMBS.1992.5760874

Filename

5760874