Extracellular measurement of acetylcholine receptor function in microcircuit-coupled myoblasts

Author

Quong, Judy N. ; Stenger, David A. ; Bryant, Howard ; Fare, Thomas L.

Author_Institution

George Washington Univ., Washington, DC, USA

Volume

1

fYear

1992

fDate

Oct. 29 1992-Nov. 1 1992

Firstpage

115

Lastpage

116

Abstract

An electrophysiological measurement principle for long-term, non-invasive monitoring of acetylcholine receptor function is described. Measurements depend on the ability to record agonist-induced depolarizations of clonal myoblasts that form high impedance seals with extracellular microcircuit electrodes. The extracellular potentials are shown to be completely eliminated by α-bungarotoxin. The technique appears promising for a variety of non-invasive electrophysiological biosensing applications.

Keywords

bioelectric potentials; biomedical measurement; biomembrane transport; biosensors; electric sensing devices; microelectrodes; molecular biophysics; patient monitoring; proteins; toxicology; α-bungarotoxin; acetylcholine receptor function; agonist-induced depolarization; clonal myoblasts; electrophysiological measurement principle; extracellular measurement; extracellular microcircuit electrodes; extracellular potentials; high impedance seals; long term noninvasive monitoring; microcircuit-coupled myoblasts; noninvasive electrophysiological biosensing; Biosensors; Facsimile;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 1992 14th Annual International Conference of the IEEE

Conference_Location

Paris

Print_ISBN

0-7803-0785-2

Electronic_ISBN

0-7803-0816-6

Type

conf

DOI

10.1109/IEMBS.1992.5760883

Filename

5760883