The effect of mechanical strain on proliferation of lung cancer A549 cells and Squamous Carcinoma of Tongue Tca8113 Cells

Using FX-4000 strain unit, the proliferation of human lung adenocarcinoma A549 cells and Squamous Carcinoma of Tongue Tca8113 Cells that underwent different mechanical strain were studied. Flow Cytometer was adopted to investigate the proliferation of A549 cells and Tca8113 cells. Experimental results indicated that cell proliferation index (PI) reduced significantly after A549 cells and Tca8113 cells were subjected to square wave with 15% elongation at frequency 0.5Hz for 4h, the PI had no distinct difference for sine wave and triangle wave treated A549 cells and Tca8113 cells when compared with the control group. It was indicated that cyclic stretch could inhibit the proliferation of A549 cells and Tca8113 cells, the waveform play important roles in inhibiting proliferation.