Electroporation effect on growth of HeLa cells

Electroporation is a process of the biophysical effect on cells exposed to an external electrical field is gaining applications in medical treatments, especially to create pores through a cell membrane and allow uptake of DNA into a cell. Therefore, in the theoretical evaluation of electroporation, transmembrane potential and characteristics of the cells growth is the target of the analysis. In this study, we used cervical cancer cells (HeLa cells) to be sample for electroporation, because HeLa cells is one of the most well-known cell lines, and easy to researched by continuous harvesting of large numbers of HeLa cells for in-vitro experimental tests. In this study we demonstrate the activity of HeLa cells induced with high voltage of 2700V/cm via a pulse length of 10μs. As a summary, it was found that the HeLa cell growth rate increased up to 50% faster when applied EP incomparison to the cell without EP treatment.