Temperature-controlled electrochemical microwell platform for studying biomolecular interactions

Author

Zuliang Shen ; Sintim, Herman O. ; Semancik, Steve

Author_Institution

Dept. of Chem. & Biochem., Univ. of Maryland, College Park, MD, USA

fYear

2013

fDate

3-6 Nov. 2013

Firstpage

1

Lastpage

3

Abstract

We designed and fabricated, on a 4-inch wafer, prototype devices for proof-of-concept studies on biomolecular interactions. The platform includes eight probe sites, each consisting of one 200 nm thick platinum (Pt) heater, one 200 nm gold (Au) and two 200 nm Pt electrodes, as well as a 20 μL polydimethylsiloxane (PDMS) well for electrochemical probing of samples. The Pt heaters have the ability to increase sample temperatures up to 120 ^oC. The device can perform electrochemical binding based measurements that detect changes in methylene blue (MB)-labeled DNA attached on the Au electrodes.

Keywords

DNA; electrochemical electrodes; electrochemical sensors; microfabrication; microsensors; polymers; MB; PDMS; biomolecular interaction; electrochemical binding based measurement; electrochemical sample probing; electrode; methylene blue-labeled DNA detection; platinum heater; polydimethylsiloxane; size 200 nm; size 4 in; temperature-controlled electrochemical microwell platform; Current measurement; DNA; Electrodes; Gold; Heating; Probes; Temperature measurement;

fLanguage

English

Publisher

ieee

Conference_Titel

SENSORS, 2013 IEEE

Conference_Location

Baltimore, MD

ISSN

1930-0395

Type

conf

DOI

10.1109/ICSENS.2013.6688368

Filename

6688368