Identification and correction of substitution errors in Moleculo long reads

Moleculo DNA sequencing technology provides extremely accurate, phased, reads having an average length of over 4,000 bp. Very little is yet known about the precise characteristics of these reads. We estimate a lower bound for the single nucleotide substitution error rate of these reads, and provide probabilities for each type of substitution. We also present preliminary work on the development of an error correction algorithm for these reads which in its current implementation corrects 74,030 single nucleotide errors in a Moleculo data set obtained from Rubus idaeus `Heritage´. We also demonstrate that the pattern of substitution errors shows no significant bias with respect to the position of an error along the body of a read.