• DocumentCode
    689254
  • Title

    Depth-resolved nanoscopic single particle tracking based on fluorescence phase-shifting interferometry

  • Author

    Arbel, Eli ; Bilenca, Alberto

  • Author_Institution
    Biomed. Eng. Dept., Ben-Gurion Univ. of the Negev, Beer-Sheva, Israel
  • fYear
    2013
  • fDate
    9-14 June 2013
  • Firstpage
    1
  • Lastpage
    2
  • Abstract
    We propose and analyze the use of fluorescence phase-shifting interferometric (PSI) imaging for single particle tracking along 1 μm-depth (z) trajectories. While spatial PSI using a single camera attains a maximal twofold improvement in the z-localization precision compared to temporal PSI for <;0.1 μm2/s diffusion, temporal PSI shows a fourfold larger field-of-view.
  • Keywords
    cameras; fluorescence; image sensors; optical tracking; phase shifting interferometry; depth 1 mum; depth trajectories; depth-resolved nanoscopic single particle tracking; field-of-view; fluorescence phase-shifting interferometric imaging; fluorescence phase-shifting interferometry; maximal twofold improvement; single camera; spatial PSI; temporal PSI; z-localization precision; Cameras; Fluorescence; Nanobioscience; Particle tracking; Photonics; Trajectory;
  • fLanguage
    English
  • Publisher
    ieee
  • Conference_Titel
    Lasers and Electro-Optics (CLEO), 2013 Conference on
  • Conference_Location
    San Jose, CA
  • Type

    conf

  • Filename
    6833637
    • Link To Document
    https://search.isc.ac/dl/search/defaultta.aspx?DTC=49&DC=689254