Biofabrication and 3D localization of multilayered cellular constructs using Laser Direct-Write and Mesoscopic Fluorescent Molecular Tomography

Recent advances in tissue engineering applications demand micro-scale precision in both design and evaluation. Herein, we aim to meet these challenges through a combination of two innovative techniques: gelatin-based Laser Direct-Write (LDW) and Mesoscopic Fluorescence Molecular Tomography (MFMT). LDW was used to precisely pattern cell-loaded, alginate microbeads, in layer-by-layer fashion, to construct thick, multilayered cellular constructs. Then, MFMT was utilized to deeply assess and 3D-model cellular localization. The combination of these two techniques may provide the accuracy and precision needed for the analysis of tissue engineering in 3D.