3D silicon electrodes with built-in glass capillaries for dielectrophoretic single-cell positioning and anaylisis

Author

Luo, Y. ; Yobas, L.

Author_Institution

Dept. of Med. Eng., California Inst. of Technol., Pasadena, CA, USA

fYear

2015

fDate

21-25 June 2015

Firstpage

540

Lastpage

543

Abstract

We present microfluidic designs for dielectrophoretic positioning of single cells and their subsequent analysis based on 3D silicon electrodes featuring built-in capillaries. The designs are presented through numerical simulations as well as experimental validations showing effectiveness of dielectrophoretic forces in trapping single cells. Single-cell experiments are also demonstrated including viability assays and ionophore-mediated intracellular calcium flux assays. Seal resistance measurements between the capillaries and cells trapped, which are essential for patch-clamp assays, are reported.

Keywords

cellular biophysics; electrochemical electrodes; electrophoresis; microelectrodes; microfluidics; silicon; 3D silicon electrodes; Si; built-in glass capillaries; dielectrophoretic single-cell positioning; ionophore-mediated intracellular calcium flux assays; microfluidic designs; numerical simulations; seal resistance measurements; viability assays; Charge carrier processes; Dielectrophoresis; Electric fields; Electrodes; Glass; Silicon; Three-dimensional displays; 3D microelectrodes; Single-cell analysis; dielectrophoresis; microcapillaries;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS), 2015 Transducers - 2015 18th International Conference on

Conference_Location

Anchorage, AK

Type

conf

DOI

10.1109/TRANSDUCERS.2015.7180980

Filename

7180980