Soft polymer-based cantilever probe for AFM nanoindentation of live mammalian cells in liquid

Author

Yu, F. ; Liu, J. ; Yu, S. ; Yang, Z. ; Pan, Y. ; Gao, N. ; Zou, Q. ; Jeon, J.

Author_Institution

Dept. of ECE, Rutgers, State Univ. of New Jersey, Piscataway, NJ, USA

fYear

2015

fDate

21-25 June 2015

Firstpage

2260

Lastpage

2263

Abstract

A soft Poly(Methyl-Meth-Acrylate)(PMMA)-polymerbased V-shaped cantilever probe is proposed for atomic force microscopy (AFM), and the first well-functioning, visually-flat prototype fabricated using a simple, but novel low-cost process and bio-compatible materials is demonstrated. With the prototype, for the first time, AFM nanoindentation was performed on live mammalian cells-human cervical adenocarcinoma cells in liquid. Results obtained using the prototype were compared to those obtained using a commercial Si₃N₄ cantilever probe. Minimal or no versus severe deformation and/or distortion of the cell membrane was observed when using the prototype versus the Si₃N₄ probe.

Keywords

atomic force microscopy; biomechanics; biomembranes; cantilevers; cellular biophysics; nanoindentation; AFM nanoindentation; atomic force microscopy; biocompatible materials; cantilever probe; cell membrane; live mammalian cell-human cervical adenocarcinoma cells; live mammalian cells; soft poly(methyl-meth-acrylate)-polymer-based V-shaped cantilever probe; soft polymer-based cantilever probe; visual-flat prototype; Arrays; Cells (biology); Force; Frequency measurement; Liquids; Probes; Prototypes; AFM; Live Cells; Nanoindentation; Nanomechanical Measurements; PMMA; Polymer Cantilever Probes;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS), 2015 Transducers - 2015 18th International Conference on

Conference_Location

Anchorage, AK

Type

conf

DOI

10.1109/TRANSDUCERS.2015.7181412

Filename

7181412