Large area real-time system for screening DNA libraries

Author

Gordon, J.S. ; McGann, W. ; Daley, K. ; McConchie, L. ; Squillante, M.R.

Author_Institution

Radiation Monitoring Devices Inc., Watertown, MA, USA

Volume

39

Issue

5

fYear

1992

fDate

10/1/1992 12:00:00 AM

Firstpage

1485

Lastpage

1489

Abstract

A nuclear imaging system based on a position-sensitive photomultiplier tube (PSPMT) and scintillating fiber optics is described. A real-time, large-area imaging system has been constructed and tested using realistic phantoms and biological samples. This prototype, which has an active area of 112 cm² was found to be more than 100 times more sensitive for detection of ³²P radiation than film and provided a spatial resolution of approximately 1.7 mm. By choosing suitable fiber dimensions and packing methods on the PSPMT this system is capable of producing images over an area equal to X-ray film and with spatial resolution approaching 0.5 mm. The digital nature of the image allows a great deal of freedom in processing and displaying data, including automatic identification and location of the desired features

Keywords

DNA; biomedical equipment; position sensitive particle detectors; radioisotope scanning and imaging; scintillation counters; DNA libraries; automatic identification; biological samples; nuclear imaging system; position-sensitive photomultiplier tube; real-time system; realistic phantoms; scintillating fiber optics; spatial resolution; DNA; Imaging phantoms; Libraries; Nuclear imaging; Optical fibers; Optical imaging; Photomultipliers; Real time systems; Spatial resolution; System testing;

fLanguage

English

Journal_Title

Nuclear Science, IEEE Transactions on

Publisher

ieee

ISSN

0018-9499

Type

jour

DOI

10.1109/23.173229

Filename

173229