Microdevices for separation, accumulation, and analysis of biological micro- and nanoparticles

Author

Kentsch, J. ; Dürr, M. ; Schnelle, T. ; Gradl, G. ; Müller, T. ; Jäger, M. ; Normann, A. ; Stelzle, M.

Author_Institution

Naturwissenschaftliches und Medizinisches Inst., Reutlingen, Germany

Volume

150

Issue

2

fYear

2003

Firstpage

82

Lastpage

89

Abstract

Microfabrication and performance of a novel microsystem for separation, accumulation and analysis of biological micro- and nanoparticles is reported. Versatile chip functions based on dielectrophoresis and microfluidics were integrated to isolate particles from complex sample solutions such as serum. A bead-based assay for virus detection is proposed. Separation of micro- and sub-μm beads employing dielectrophoretic deflector and bandpass structures is demonstrated. Individual antibody coated beads with hepatitis A virus bound to their surface were trapped by negative dielectrophoresis in a field cage and analysed by fluorescence microscopy.

Keywords

biochemistry; bioelectric phenomena; biosensors; cellular biophysics; electrophoresis; fluorescence; microelectrodes; microfluidics; microorganisms; nanoparticles; optical microscopy; spectrochemical analysis; accumulation; analysis; antibody coated beads; bandpass structures; bead-based assay; biological analytes; biological microparticles; biological nanoparticles; complex sample solutions; dielectrophoresis; dielectrophoretic deflector; field cage; fluorescence microscopy; hepatitis A virus; microdevices; microfabrication; microfluidics; microsystem; negative dielectrophoresis; separation; serum; versatile chip functions; virus detection;

fLanguage

English

Journal_Title

Nanobiotechnology, IEE Proceedings -

Publisher

iet

ISSN

1478-1581

Type

jour

DOI

10.1049/ip-nbt:20031127

Filename

1260181