3-D reconstruction of 2-D crystals in real space

Author

Marabini, Roberto ; Sorzano, Carlos O S ; Matej, Samuel ; Fernández, José J. ; Carazo, José M. ; Herman, Gabor T.

Author_Institution

Escuela Politecnica Superior, Univ. Autonoma de Madrid, Spain

Volume

13

Issue

4

fYear

2004

fDate

4/1/2004 12:00:00 AM

Firstpage

549

Lastpage

561

Abstract

A new algorithm for three-dimensional reconstruction of two-dimensional crystals from projections is presented, and its applicability to biological macromolecules imaged using transmission electron microscopy (TEM) is investigated. Its main departures from the traditional approach is that it works in real space, rather than in Fourier space, and it is iterative. This has the advantage of making it convenient to introduce additional constraints (such as the support of the function to be reconstructed, which may be known from alternative measurements) and has the potential of more accurately modeling the TEM image formation process. Phantom experiments indicate the superiority of the new approach even without the introduction of constraints in addition to the projection data.

Keywords

biological techniques; biology computing; electron diffraction crystallography; image reconstruction; iterative methods; macromolecules; molecular biophysics; transmission electron microscopy; Fourier transforms; algebraic reconstruction techniques; biochemical research; biological macromolecules imaging; image formation process; iterative method; real space; three-dimensional crystal reconstruction; transmission electron microscopy; two-dimensional crystals; Assembly; Crystals; Electron microscopy; Image reconstruction; Iterative algorithms; Iterative methods; Proteins; Reconstruction algorithms; Three dimensional displays; Transmission electron microscopy; Algorithms; Bacillus Phages; Capsid Proteins; Computer Graphics; Crystallography; Image Enhancement; Image Interpretation, Computer-Assisted; Imaging, Three-Dimensional; Information Storage and Retrieval; Macromolecular Substances; Microscopy, Electron; Molecular Conformation; Pattern Recognition, Automated; Phantoms, Imaging; Reproducibility of Results; Sensitivity and Specificity; Signal Processing, Computer-Assisted;

fLanguage

English

Journal_Title

Image Processing, IEEE Transactions on

Publisher

ieee

ISSN

1057-7149

Type

jour

DOI

10.1109/TIP.2003.822620

Filename

1284391