Semi-automatic segmentation of vascular network images using a rotating structuring element (ROSE) with mathematical morphology and dual feature thresholding

A method for measuring the spatial concentration of specific categories of vessels in a vascular network consisting of vessels of several diameters, lengths, and orientations is demonstrated. It is shown that a combination of the mathematical morphology operation, opening, with a linear rotating structuring element (ROSE) and dual feature thresholding can semi-automatically segment categories of vessels in a vascular network. Capillaries and larger vessels (arterioles and venules) are segmented here in order to assess their spatial concentrations. The ROSE algorithm generates the initial segmentation, and dual feature thresholding provides a means of eliminating the nonedge artifact pixels. The subsequent gray-scale histogram of only the edge pixels yields the correct segmentation threshold value. This image processing strategy is demonstrated on micrographs of vascular casts. By adjusting the structuring element and rotation angles, it could be applied to other network structures where a segmentation by network component categories is advantageous, but where the objects can have any orientation