مقايسۀ تأثير 12 هفته تمرين تناوبي با شدت بالا و متوسط بر پري ليپين 3 بافت چربي احشايي و مقاومت به انسولين در رت‌هاي نر ديابتي نوع 2

A Comparison of the Effect of 12 Weeks of Interval Training with High and Moderate Intensity on Perilipin 3 of Visceral Adipose Tissue and Insulin Resistance in Type 2 Diabetic Male Rats

ﻫﺪف از ﭘﮋوﻫﺶ ﺣﺎﺿﺮ ﻣﻘﺎﻳﺴﺔ ﺗﺄﺛﻴﺮ 12 ﻫﻔﺘﻪ ﺗﻤﺮﻳﻦ ﺗﻨﺎوﺑﻲ ﺑﺎ ﺷﺪت ﺑﺎﻻ و ﻣﺘﻮﺳﻂ ﺑﺮ ﭘﺮيﻟﻴﭙﻴﻦ3 )PLIN3( ﺑﺎﻓﺖ ﭼﺮﺑﻲ  اﺣﺸﺎﻳﻲ و ﻣﻘﺎوﻣﺖ ﺑﻪ اﻧﺴﻮﻟﻴﻦ رتﻫﺎي ﻧﺮ دﻳﺎﺑﺘﻲ ﻧﻮع 2 ﺑﻮد. در اﻳﻦ ﻣﻄﺎﻟﻌﻪ 24 ﺳﺮ رت ﻧﺮ دﻳﺎﺑﺘﻲ ﻧﻮع 2 ﺑﻪﺻﻮرت ﺗﺼـﺎدﻓﻲ ﺑـﻪ ﺳـﻪ ﮔـﺮوه ﺗﻤﺮﻳﻦ ﺗﻨﺎوﺑﻲ ﺑﺎ ﺷﺪت ﻣﺘﻮﺳﻂ )MIIT(، ﺗﻤﺮﻳﻦ ﺗﻨﺎوﺑﻲ ﺷﺪت ﺑﺎﻻ )HIIT( و ﻛﻨﺘـﺮل ﺗﻘﺴـﻴﻢ ﺷـﺪﻧﺪ. ﺗﻤﺮﻳﻨﺎت ﺗﻨﺎوﺑﻲ )MIIT ،HIIT ( ﺑﻪ ﻣﺪت 12 ﻫﻔﺘﻪ و 5 ﺟﻠﺴﻪ در ﻫﻔﺘﻪ اﺟﺮا ﺷﺪ. ﭘﺮوﺗﻜﻞ MIIT ﺷﺎﻣﻞ اﺟﺮاي 13 وﻫﻠﻪ ﻓﻌﺎﻟﻴﺖ ﭼﻬﺎردﻗﻴﻘﻪاي ﺑﺎ ﺷﺪت 65-70 درﺻﺪ VO2max و ﭘﺮوﺗﻜﻞ HIIT ﺷﺎﻣﻞ اﺟﺮاي 10 وﻫﻠﻪ ﻓﻌﺎﻟﻴﺖ ﭼﻬﺎردﻗﻴﻘﻪاي ﺑﺎ ﺷﺪت 85-90 درﺻﺪ VO2max ﺑﺎ دورهﻫﺎي اﺳﺘﺮاﺣﺘﻲ ﻓﻌﺎل دودﻗﻴﻘﻪاي ﺑﻮد. از روش وﺳﺘﺮن ﺑﻼت ﺑﺮاي اﻧﺪازهﮔﻴﺮي ﺳﻄﻮح ﭘﺮوﺗﺌﻴﻨﻲ PLIN3 اﺳﺘﻔﺎده ﺷﺪ. از آزﻣﻮنﻫﺎي ANOVA و آزﻣﻮن ﺗﻮﻛﻲ ﺑﺮاي ﺗﺤﻠﻴﻞ دادهﻫﺎ اﺳﺘﻔﺎده ﺷﺪ. ﻧﺘﺎﻳﺞ ﺗﺤﻠﻴﻞ دادهﻫﺎ ﺑﻴﺎﻧﮕﺮ ﻛﺎﻫﺶ ﻣﻌﻨﺎدار PLIN3در ﮔﺮوهﻫﺎي HIIT وMIIT ﻧﺴﺒﺖ ﺑـﻪ ﮔـﺮوه ﻛﻨﺘـﺮل ﺑـﻮد )0/001=P(، ﺑﺎ اﻳﻦ ﺣﺎل، ﺗﻔﺎوت ﻣﻌﻨﺎداري ﺑﻴﻦ ﮔﺮوهﻫﺎي ﺗﻤﺮﻳﻨﻲ وﺟﻮد ﻧﺪاﺷﺖ )0/274=P(. ﻫﻤﭽﻨﻴﻦ، ﻫﺮ دو ﭘﺮوﺗﻜﻞ HIIT و MIIT ﺑﻪ ﻛﺎﻫﺶ ﻣﻌﻨﺎداري در ﺳﻄﻮح ﺳﺮﻣﻲ ﮔﻠﻮﻛﺰ ﻣﻨﺠﺮ ﺷﺪﻧﺪ )0/001=P(، درﺣﺎﻟﻲﻛﻪ اﻧﺴﻮﻟﻴﻦ ﺳﺮﻣﻲ ﻛﺎﻫﺶ و ﺗﺄﺛﻴﺮ ﻣﻌﻨﺎداري ﺑﺮ ﻣﻘﺎوﻣﺖ ﺑﻪ اﻧﺴﻮﻟﻴﻦ ﻧﺪاﺷﺘﻨﺪ )0/05˃P(. ﻫﻤﭽﻨﻴﻦ، ﺗﻔﺎوت ﻣﻌﻨﺎداري ﺑﻴﻦ ﮔﺮوهﻫﺎي ﺗﻤﺮﻳﻨﻲ در ﺗﻐﻴﻴﺮات اﻧﺴﻮﻟﻴﻦ، ﮔﻠﻮﻛﺰ و ﻣﻘﺎوﻣﺖ ﺑﻪ اﻧﺴﻮﻟﻴﻦ وﺟﻮد ﻧﺪاﺷﺖ )0/05˃P(. ﻳﺎﻓﺘﻪﻫﺎي ﭘﮋوﻫﺶ ﺣﺎﺿﺮ ﻧﺸﺎن داد ﻛﻪ ﻫﺮ دو ﭘﺮوﺗﻜﻞ HIIT و MIIT ﻣﻮﺟﺐ ﻛﺎﻫﺶ ﭼﺸﻤﮕﻴﺮ PLIN3 ﭼﺮﺑﻲ اﺣﺸﺎﻳﻲ و ﺑﻬﺒﻮد ﻣﺘﺎﺑﻮﻟﻴﺴﻢ ﮔﻠﻮﻛﺰ در رتﻫﺎي دﻳﺎﺑﺘﻲ ﻧﻮع 2 ﻣﻲﺷﻮﻧﺪ. ﺑﻨﺎﺑﺮاﻳﻦ، ﺑﻪﻧﻈﺮ ﻣﻲرﺳﺪ ﻛﻪ ﺗﻐﻴﻴﺮات PLIN3 ﭼﺮﺑﻲ اﺣﺸﺎﻳﻲ ﻣﺴﺘﻘﻞ از ﺷﺪت ﻓﻌﺎﻟﻴﺖ ورزﺷﻲ اﺳﺖ.

The aim of this study was to compare the effect of 12 weeks of interval training with high and moderate intensity on perilipin 3 (PLIN3) of visceral adipose tissue and insulin resistance in type 2 diabetic male rats. In this study, 24 male rats with type 2 diabetes were randomly divided into three groups: interval training with moderate intensity (MIIT), interval training with high intensity (HIIT) and control. Interval training (HIIT, MIIT) was applied for 12 weeks, 5 sessions per week. The MIIT protocol included 13 four-min. bouts of activity with intensity of 65-70% vo2max and the HIIT protocol included 10 four-min. bouts of activity with intensity of 85-90% vo2max with 2-minute active rest periods. Western Blot method was used to measure PLIN3 protein levels. ANOVA and Tukey's test were used for data analysis. The results indicated a significant decrease in PLIN3 in the HIIT and MIIT groups compared with the control group (P=0.001). However, there was no significant difference between the training groups (P=0.274). Also, both HIIT and MIIT protocols significantly decreased serum levels of glucose (P=0.001), while they had no significant effects on serum insulin and insulin resistance (P˃0.05). There was no significant difference between training groups in insulin, glucose and insulin resistance changes (P˃0.05). The findings of this study showed that both HIIT and MIIT protocols drastically reduced PLIN3 of visceral adipose and improved glucose metabolism in type 2 diabetic rats. Therefore, it seems that PLIN3 changes in visceral adipose are independent of the training intensity.