ﺗﺄﺛﯿﺮ دوازده ﻫﻔﺘﻪ رژﯾﻢ ﻏﺬاﯾﯽ ﭘﺮﭼﺮب و ﺗﻤﺮﯾﻦ ورزﺷﯽ ﺑﺎ ﺷﺪت ﻣﺘﻮﺳﻂ ﺑﺮ ﻣﺴﯿﺮ 15-KLF / 3-KLF / ﻓﻮرﯾﻦ / 12-CTRP ﺑﺎﻓﺖ ﭼﺮﺑﯽ اﺣﺸﺎﯾﯽ رتﻫﺎي ﻧﺮ

The Effect of 12 Weeks of High Fat Diet and Moderate Intensity Training on the KLF-15 / KLF-3 / Furin / CTRP-12 Pathway of Visceral Adipose Tissue in Male Rats

از ﺟﻤﻠﻪ ﻓﺎﮐﺘﻮرﻫﺎي ﻣﻘﺎﺑﻠﻪﮐﻨﻨﺪه ﺑﺎ رﯾﺴﮏ ﻓﺎﮐﺘﻮرﻫﺎي ﻣﺘﺎﺑﻮﻟﯿﮑﯽ، 12-CTRP اﺳﺖ. ﭼﺎﻗﯽ ﺑﺎ ﺗﻨﻈﯿﻢ ﻣﺜﺒﺖ ﻓﺎﮐﺘﻮرﻫﺎي ﻓﻮرﯾﻦ و 3-KLF و ﺗﻨﻈﯿﻢ ﻣﻨﻔﯽ 15-KLF در ﻣﻬﺎر 12-CTRP ﻧﻘﺶ ﺑﺴﺰاﯾﯽ دارد. ﺗﻤﺮﯾﻦ ورزﺷﯽ ﺑﺮ ﭘﯿﺎمرﺳﺎﻧﯽ ﺳﻠﻮلﻫﺎي ﭼﺮﺑﯽ ﺗﺄﺛﯿﺮﮔﺬار اﺳﺖ. ازاﯾﻦرو ﻫﺪف ﻣﻄﺎﻟﻌﮥ ﺣﺎﺿﺮ ﺑﺮرﺳﯽ ﺗﺄﺛﯿﺮ 12 ﻫﻔﺘﻪ ﺗﻤﺮﯾﻦ ورزﺷﯽ ﺑﺎ ﺷﺪت ﻣﺘﻮﺳﻂ )MICT( ﺑﺮ 12-CTRP، ﻓﻮرﯾﻦ، 3-KLF و 15-KLF ﺑﺎﻓﺖ ﭼﺮﺑﯽ اﺣﺸﺎﯾﯽ رتﻫﺎي ﻧﺮ ﭼﺎق ﺑﻮد. 15 ﺳﺮ رت ﻧﺮ ﻧﮋاد وﯾﺴﺘﺎر، اﺑﺘﺪا ﺑﻪ 2 ﮔﺮوه ﺗﻐﺬﯾﻪ ﺑﺎ ﻏﺬاي ﭘﺮﭼﺮب )10 ﺳﺮ( و ﺗﻐﺬﯾﻪ ﺑﺎ ﻏﺬاي اﺳﺘﺎﻧﺪارد )5 ﺳﺮ( ﺗﻘﺴﯿﻢ ﺷﺪﻧﺪ. ﭘﺲ از 12 ﻫﻔﺘﻪ، ﻣﺠﺪداً ﮔﺮوه ﺗﻐﺬﯾﻪ ﺑﺎ ﻏﺬاي ﭘﺮﭼﺮب ﺑﻪ دو ﮔﺮوه MICT و HFD ﺗﻘﺴﯿﻢ ﺷﺪﻧﺪ. ﺑﺮﻧﺎﻣﮥ ﺗﻤﺮﯾﻨﯽ ﺑﻪ ﻣﺪت 12 ﻫﻔﺘﻪ، 5 ﺟﻠﺴﻪ در ﻫﻔﺘﻪ و ﺑﺎ ﺷﺪت 60-50 درﺻﺪ ﺣﺪاﮐﺜﺮ ﺳﺮﻋﺖ اﺟﺮا ﺷﺪ. ﻣﻘﺎدﯾﺮ ﻣﺘﻐﯿﺮﻫﺎ ﺑﻪ روش اﻻﯾﺰا اﻧﺪازهﮔﯿﺮي ﺷﺪ. ﺗﺠﺰﯾﻪوﺗﺤﻠﯿﻞ دادهﻫﺎ ﺑﺎ آزﻣﻮن آﻣﺎري ANOVA و ﻧﺮماﻓﺰار ﭘﺮﯾﺰم 5 اﻧﺠﺎم ﮔﺮﻓﺖ. ﻧﺘﺎﯾﺞ ﻣﻄﺎﻟﻌﮥ ﺣﺎﺿﺮ ﻧﺸﺎن داد ﮐﻪ HFD ﺳﺒﺐ اﻓﺰاﯾﺶ ﻣﻌﻨﺎدار ﻣﻘﺎدﯾﺮ ﻓﻮرﯾﻦ و 3-KLF ﺑﺎﻓﺖ ﭼﺮﺑﯽ اﺣﺸﺎﯾﯽ ﺷﺪ )0/05≤P(. 12 ﻫﻔﺘﻪ ﺗﻤﺮﯾﻦ MICT ﺳﺒﺐ ﮐﺎﻫﺶ ﻣﻌﻨﺎدار ﻣﻘﺎدﯾﺮ ﻓﻮرﯾﻦ، -KLF 3 و اﻓﺰاﯾﺶ ﻣﻌﻨﺎدار 15-KLF ﻧﺴﺒﺖ ﺑﻪ ﮔﺮوه HFD ﺷﺪ )0/05≤P(. ﺗﻐﯿﯿﺮات 12-CTRP ﺑﯿﻦ ﮔﺮوهﻫﺎي ﻣﺨﺘﻠﻒ ﻣﻌﻨﺎدار ﻧﺒﻮد )0/386=F=1.032 , P(. 12 ﻫﻔﺘﻪ MICT ﻣﺠﺰا از ﺗﻐﯿﯿﺮات 12-CTRP، در ﮐﺎﻫﺶ رﯾﺴﮏ ﻓﺎﮐﺘﻮرﻫﺎي ﺳﻠﻮﻟﯽ ﻣﻀﺮ ﺑﺎﻓﺖ ﭼﺮﺑﯽ )ﻓﻮرﯾﻦ و 3-KLF( و اﻓﺰاﯾﺶ ﻓﺎﮐﺘﻮرﻫﺎي ﻣﻔﯿﺪ ﺑﺎﻓﺖ ﭼﺮﺑﯽ )15-KLF( ﻣﺆﺛﺮ اﺳﺖ. ازاﯾﻦرو ﭘﯿﺸﻨﻬﺎد ﻣﯽﺷﻮد اﻓﺮاد در ﻣﻌﺮض ﭼﺎﻗﯽ ﺑﺮاي ﺳﺎزﮔﺎري ﺑﻬﺘﺮ ﺳﻠﻮﻟﯽ، ﺑﻪ ﺗﻤﺮﯾﻨﺎت ورزﺷﯽ ﺑﺎ ﻣﺪت زﻣﺎن ﻃﻮﻻﻧﯽﺗﺮ رو ﺑﯿﺎورﻧﺪ.

One of the factors that counteracts with risk factors of metabolic disorder is CTRP-12. Obesity plays an important role in the downregulation of CTRP-12 by positive regulation of furin and KLF-3 factors and negative regulation of KLF-15. Training affects adipocytes signaling. Therefore, the aim of the present study was to investigate the effect of 12 weeks of moderate intensity continuous training (MICT) on the KLF-15 / KLF-3 / furin / CTRP-12 of visceral adipose tissue in male obese rats. 15 male Wistar rats were divided into two groups: high fat diet (n=10) and standard diet (n=5). After 12 weeks, the high fat diet group was again divided into two groups: MICT and HFD. Training program was performed for 12 weeks, 5 sessions per week with intensity of 50-60% of maximum speed. The values of variables were measured by ELISA method. Data were analyzed using ANOVA and Prism 5 software. The results of this study showed that HFD significantly increased the amount of furin and KLF-3 in visceral adipose tissue (P≤0.05). The 12 weeks of MICT significantly decreased the levels of furin, KLF-3 and significantly increased KLF-15 compared with the HFD group (P≤0.05). Changes in CTRP-12 were not significant between groups (F = 1.032, P = 0.386). 12 weeks of MICT, apart from changes in CTRP-12, was effective in decreasing the risk of adipose tissue cellular factors (furin and KLF-3) and in increasing the beneficial factors of adipose tissue (KLF-15). Therefore, it is recommended that those exposed to obesity should get longer training sessions for better cellular adaptation.